Monday, October 15, 2012

1210.3378 (S. G. J. Mochrie et al.)

Unwinding and rewinding the nucleosome inner turn: Force dependence of
the kinetic rate constants
   [PDF]

S. G. J. Mochrie, A. H. Mack, D. J. Schlingman, R. Collins, M. Kamenetska, L. Regan
A simple model for the force-dependent unwinding and rewinding rates of the nucleosome inner turn is constructed and quantitatively compared to the results of recent measurements [A. H. Mack et al., J. Mol. Biol. 423, 687 (2012)]. First, a coarse-grained model for the histone-DNA free energy landscape that incorporates both an elastic free energy barrier and specific histone-DNA bonds is developed. Next, a theoretical expression for the rate of transitions across a piecewise linear free energy landscape with multiple minima and maxima is presented. Then, the model free energy landscape, approximated as a piecewise linear function, and the theoretical expression for the transition rates are combined to construct a model for the force-dependent unwinding and re-winding rates of the nucleosome inner turn. Least-mean-squares fitting of the model rates to the rates observed in recent experiments rates demonstrates that this model is able to well describe the force-dependent unwinding and rewinding rates of the nucleosome inner turn, observed in the recent experiments, except at the highest forces studied, where an additional ad hoc term is required to describe the data, which may be interpreted as an indication of an alternate high-force nucleosome disassembly pathway, that bypasses simple unwinding. The good agreement between the measurements and the model at lower forces demonstrates that both specific histone-DNA contacts and an elastic free energy barrier play essential roles for nucleosome winding and unwinding, and quantifies their relative contributions.
View original: http://arxiv.org/abs/1210.3378

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